Journal: BMC Cancer

Article Title: Targeting tissue factor on tumour cells and angiogenic vascular endothelial cells by factor VII-targeted verteporfin photodynamic therapy for breast cancer in vitro and in vivo in mice

doi: 10.1186/1471-2407-10-235

Figure Lengend Snippet: TF is expressed on tumour cells and VEGF-stimulated HUVECs but not on unstimulated HUVECs . A . TF expression was detected on breast cancer cell lines but not on CHO-K1 cells by flow cytometry using mouse Icon (mfVII/hIgG1 Fc) protein. B . Selective expression of TF on VEGF-stimulated HUVECs but not on unstimulated HUVECs was observed and photographed under confocal microscope. HUVECs were stained for TF expression by mouse Icon or anti-HTF antibody (FITC, green) and then by anti-human CD31 PE (red) to verify that TF-expressing cells were of vascular endothelial origin. Results in A and B are representative of two experiments.

Article Snippet: Chinese Hamster Ovary K1 (CHO-K1, ATCC) cells were grown in F12 medium, murine breast cancer EMT6 and human embryonic kidney 293 cells (kindly provided by Dr. Albert Deisseroth during his tenure at Yale University) in DMEM and human breast cancer MDA-MB-231 cells in RPMI1640 medium supplemented with 10% heat inactivated fetal bovine serum (FBS) (Invitrogen) and 1:100 antibiotics (Sigma).

Techniques: Expressing, Flow Cytometry, Microscopy, Staining

Journal: BMC Cancer

Article Title: Targeting tissue factor on tumour cells and angiogenic vascular endothelial cells by factor VII-targeted verteporfin photodynamic therapy for breast cancer in vitro and in vivo in mice

doi: 10.1186/1471-2407-10-235

Figure Lengend Snippet: fVII targeting improves the selectivity of VP PDT . A . Incubation time of fVII-VP and free VP affected the effect of fVII-tPDT and ntPDT (2 μM, 689 nm laser at 60 J/cm 2 ) on killing MDA-MB-231 cancer cells. However, VP alone without laser irradiation (VP alone) did not have an effect on killing the cancer cells. B . Comparison of fVII-tPDT and ntPDT (689 nm laser at 60 J/cm 2 ) for side effects on non-TF-expressing CHO-K1 cells as a normal cell line. C . VP PDT (fVII-tPDT or ntPDT with 0.5 or 5 μM VP at 60 J/cm 2 ) for the non-TF expressing 293 line as a normal cell line control. D and E . fVII-tPDT and ntPDT (689 nm laser at 36 J/cm 2 ) for HUVEC with or without induction of TF expression by VEGF, as seen in Figure 2B. Results in A-D are representative of two experiments.

Article Snippet: Chinese Hamster Ovary K1 (CHO-K1, ATCC) cells were grown in F12 medium, murine breast cancer EMT6 and human embryonic kidney 293 cells (kindly provided by Dr. Albert Deisseroth during his tenure at Yale University) in DMEM and human breast cancer MDA-MB-231 cells in RPMI1640 medium supplemented with 10% heat inactivated fetal bovine serum (FBS) (Invitrogen) and 1:100 antibiotics (Sigma).

Techniques: Incubation, Irradiation, Comparison, Expressing, Control

Journal: Cell reports

Article Title: Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans

doi: 10.1016/j.celrep.2020.108425

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse anti-Nkx6.1 (1:100) , DSHB , Cat#: F55A10 RRID: AB_532378.

Techniques: Recombinant, Staining, Mouse Assay, Mutagenesis, RNAscope, Plasmid Preparation, Software, Microscopy